PJB-2017-857
BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF BRASSICA NAPUS ADVANCED LINES USING SSR MARKERS
Safi ullah
Abstract
The oil quality of Brassica napus seed is determined by its fatty acid profile. Studies were undertaken to determine the molecular characterization of 45 advanced lines of Brassica napus using 11 Simple Sequence Repeat (SSR) markers and investigate the fatty acid profile of these selected lines using GC/MS. The molecular studies revealed that 8 primers out of 11 showed polymorphic bands whose size ranged from 100-250bp. The highest frequency was shown by BRMS-287, which was detected in all selected lines except line number 18 (Dunkled X E7). Bivariate data (presence=1, absence=0) matrix was generated and relative distance was calculated using R-software with hamming procedure. The cluster analysis revealed that there is less dissimilarity in 45 lines and relative distance ranged from 0-10, which can be efficiently managed in breeding program for the development of nutritionally better quality local genotypes. The fatty acid composition among F6 population values indicate that contents of oleic, linoleic and linolenic acid ranged from 31.09-65.50%, 3.01-28.00% and 0-15.00%, respectively. The desirable erucic acid (<5%) was recorded in majority of lines and highest erucic acid (18%) content was observed in line number 7 (Dunkled X E4). The desirable (<7%) saturated fatty acids (Palmatic and Stearic acid) were reported in 74% of lines, while the content of eicosenoic acid ranged from 1.2% to 36.31%. The correlation studies showed that erucic acid content was negatively correlated with all observed fatty acids except linolenic and stearic acid showing positive correlation. From the present study, it is recommended that lines with modified fatty acid profile and higher yield may be approved for commercial cultivation in farmer
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