PJB-2017-863
MOLECULAR CHARACTERIZATION OF DIFFERENT WHEAT ACCESSIONS/LINES FOR LEAF RUST RESISTANCE USING SSR MARKERS
Aqsa Farid
Abstract
MOLECULAR CHARACTERIZATION OF DIFFERENT WHEAT ACCESSIONS/LINES FOR LEAF RUST RESISTANCE USING SSR MARKERS AQSA FARID1, IQBAL MUNIR1, AQIB IQBAL1, SAFI ULLAH1 AND NAEMA GUL2 Leaf rust is the most destructive disease of wheat (Triticum aesttivuma) worldwide. The molecular characterization of 30 wheat accessions/lines were investigated using 13 polymorphic Simple Sequence Repeats (SSRs) primers i.e., Lr9, Lr13.Lr19, Lr24, Lr26, Lr28, Lr32, Lr34, Lr35, Lr36, Lr37, Lr39 and Lr46. Six SSRs primer produce reproducible bands. The highest frequency (96.7%) was observed for Lr32, as it was detected in 29 accessions that cause resistance against wheat rust, while the lowest frequency (36.7%) was detected for Lr 28, as it was observed in 11 accessions and was absent in other remaining lines. However, all the six resistant genes were identified in two accessions (012012 and 018770) which is 6.7% of the studied population. In addition, the similarity coefficient based on six primers ranged from 0.26 to 1.00, there is less dissimilarity in 30 accessions which are efficiently managed in breeding program. The existing research found that SSR markers could differentiate and characterize all the genotypes of studied accessions so more screened primers could be used for study. Furthermore, the identification of six rust resistant genes in wheat accessions will help in accelerating the breeding program in future and reducing yield losses to this disease. Key words: Wheat, Leaf rust resistance, Microsatellites, SSR markers.
To Cite this article:
Download
