PJB-2017-872
CRISPR Cas9 Nuclease recruitment for targeted mutagenesis using oligo SgRNA in Soybean (Glycine max L.).
Noor al Amin
Abstract
A modern era of genome engineering via targeted mutagenesis is now bringing further innovations in the field of developmental biology, Plant biology and biomedicines. RNA-guided endonucleases, Clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9 system has been proved as most powerful and accurate tool to study the mechanism of genome screening and engineering. We report the collection of resources that can redirect and ensures the flexibility of targeted genomic alteration in eukaryotes. Single guide RNA can be recruited more precisely and efficiently to become a powerful tool in genome manipulation overcoming a broader aspect of synthetic biology. CRISPR P2.0 is a local tool known for the optimization of single guide RNA in plant research. The overview of all possible single gRNA on-targeted mutagenesis in any user-provided sequence has been demonstrated with specified protospacer adjacent motif (PAM) in soybean seed. We hope to deliver enough confidence for our readers to get started and design your own graphical genome model which could significantly improve your research.
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