PJB-2017-873
Molecular Characterization of Olives Genotypes Through DNA Finger Printing
Fahad Ali Shah
Abstract
The proposed study was conducted to molecularly characterize the 20 unknown and 5 known genotypes of olive collected from swabi research station in 2016. Three SSR markers were used. The PCR reactions were performed with these SSR markers. The PCR products were run on 3% agarose gel. The number as well as the size of alleles was measured. Both homozygous as well as heterozygous bands of alleles were obtained. DCAO9 gave the maximum number of alleles while the minimum number of alleles was obtained when the SSR UDO99-012 was used. Genetic diversity was found to be maximum in case of the primer DCAO9 while UDO-99-012 resulted in minimum gene diversity. Allele richness was also the greatest when DCA09 was used while the lowest allele richness was recorded in case of UDO-99-012. FIT values ranged from 0.029 to 0.369. Genetic diversity between the populations was also observed. The results of this study revealed a low genetic diversity among the selected genotypes.
To Cite this article:
Download
