PJB-2017-882
DEVELOPMENT AND OPTIMIZATION OF A COST-EFFECTIVE PROPAGATION IN VITRO METHODOLOGY FOR GLADIOLUS
Rana Muhammad Mateen
Abstract
Gladiolus is a non-native, costly but important flowering plant in South Asia. Considering its lower production rate, and economic importance, micropropagation technology was optimized in this study to establish a protocol for pathogen free clonal genotypes. Taken the results together, best response was observed at 27 °C as growing temperature, table sugar (sucrose) as a carbon source, apical meristem of 3 mm for shoot formation as an explant. Whereas, the best plant regeneration was observed for shoot apical meristem (i.e. 96% within 7.8 days of inoculation) using Murashige and Skoog (MS) basal medium with 1.0 mg/l BAP and 0.5 mg/l Kinetin. The best shoot multiplication was 98% in 18.2 days of inoculation using MS media with 1.0 mg/l BAP only. For root induction, MS media with 1.0 mg/l NAA and 2.0 mg/l IBA gave the best results (i.e. 100 % in 6.8 days). The best supporting agent was found to be cotton swab while the best media for hardening and acclimatization of well-developed plants was a combination of sand, soil, and peat (1:1:1), leading to 80% survival of the plants. The results obtained in this study may better help grow gladiolus plants on an industrial scale with a lower cost of production.
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