PJB-2017-891
Construction of Expression Vectors of Melon Resistance Gene Fom-2 and Genetic Transformation
Luan Feishi
Abstract
In this study, we constructed a positive and negative expression vector using Melon Wilt Resistance Related Gene Fom-2, and transformed it into melon by the pollen tube pathway. In the field, 52 Kan resistant M4-45 plants and 84 Kan resistant MR-1 plants were transformed with the Antisense Vector, and the conversion rates were 3.28% and 4.26%, respectively. The results of PCR showed that antisense vectors were integrated into the genome of Cucumis melo. The results of a special inoculation test of Fusarium oxysporum melonis showed that the incidence of Fusarium Wilt in transgenic plants was 84% and that in non-transgenic plants was 96%. The content of chitinase, polyphenol oxidase and peroxidase increased with bacterial treatment time in the transgenic plants and non-transgenic plants, and the activities of these three enzymes were higher than that of non-transgenic plants.
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