PJB-2017-907
A BIOSYNTHESIS METHOD TO PRODUCE RESVERATROL IN SACCHAROMYCES CEREVISIAE WITH SECRETION EXPRESSION OF GRAPE ANTI-OXIDANT RESVERATROL SYNTHASE GENE DURING WINE FERMENTATION: SECRETION EXPRESSION OF RS WITH YEAST
YANGBO SONG
Abstract
Resveratrol (Res), a stilbenic compound, recently has become the focus of a number of studies in medicine and plant physiology. While Saccharomyces cerevisiae (S. cerevisiae) has become a popular host for production of non-native compounds. As the last and key enzyme of biosynthesis pathway, the amount and expression level of Resveratrol Synthase (RS) impact the production of Res. In this study, a signal peptide sequence, secreted by α-factor of S. cerevisiae, was connected with the RS gene, resulting a pYCα secretory plasmid construction. The RS gene was secreted expression in S. cerevisiae successfully. Subsequently, a white wine fermentation was carried out by the recombinant strain. The Res concentration of the white wine fermented by the recombinant strain was 36% higher than the control. This has great interests for establishing a controllable way to produce RS and lays the foundation for exploring the Res biosynthesis method.
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