PJB-2017-916
INVESTIGATION REGARDING BACTERIAL LEAF BLIGHT OF RICE THROUGH VARIOUS DETECTION TOOLS AND ITS IMPACT ON CROP YIELD IN PUNJAB, PAKISTAN
Rozina Shaheen
Abstract
Bacterial blight instigated by Xanthomonas oryzae pv. oryzae (Xoo) is an utmost imperative bacterial disease in rice crop and is acknowledged to cause heavy economic losses. In current investigation the rice leaf samples from infested regions were screened for Xoo linked incidence and 17 isolates were identified based on biochemical tests (Gram staining and KOH) and molecular examines (PCR). Moreover, the percentage diseases incidence and weight loss of 1000 grain of each sample from four zones of rice production were recorded. Both biochemical test exhibited red stained and rod like Gram –ve bacteria. In addition, the molecular recognition by means of a 16S rRNA universal primer revealed DNA amplification in 15 out of 17 isolates which confirmed the pathogen as “Xoo”. The data assessed for disease incidence in all investigated districts ranged between 70.12%- 49.23%. While, the maximum and minimum weight losses of 17.84% and 11.17% from Sialkot and Narowal were recorded correspondingly. The employment of such tools for Xoo detection and its impact on crop yield are debated in this investigation.
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