PJB-2018-38
EX-SITU CONSERVATION OF HAPLOPHYLLUM GILESII: A CRITICALLY ENDANGERED AND NARROW ENDEMIC SPECIES OF THE KARAKORAM RANGE
ROBINA KAUSAR
Abstract
The present study aimed to develop efficient in-vitro methods for the rapid micro-propagation via callus and axillary buds for ex-situ conservation of Haplophyllum gilesii (Hemsel) C.C. Townsend, a critically endangered, narrow endemic species of Gilgit Baltistan region of Pakistan. Basal MS media with different combinations of growth regulators (IBA, BAP, NAA, GA3 and kinetin) were used for root initiation, shoot multiplication, callus induction and regeneration. MS medium supplemented with 2.5mgL-1 BAP showed maximum number (6.8) of shoots after 35 days of culture. Half strength MS media with 1.5mgL-1 GA3 and 0.4mgL-1 kinetin was most effective for induction of healthy roots. BAP (2.5mgL-1), kinetin (0.25mgL-1) and NAA (2.5mgL-1) showed efficient callus initiation and growth while highest regeneration was observed at the medium having 1mgL-1 IBA. Acclimatized plantlets exhibited 100% survival rate for one week, 60% after one month while 45% plants survived for 5 month in pots. The protocol developed here can be effectively used for ex-situ and in-situ conservation and mass multiplication production of H. gilesii plantlets as well as callus to get benefit from this plant without posing threats to wild populations. Molecular composition and secondary metabolic constituents of this plant still needs investigations.
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