PJB-2018-56
Antigenic analysis of PVY- EG - coat protein and predicted epitopes
Seham Abdel-Shafi1
Abstract
Isolated Potato virus Y (PVY- EG) obtained from naturally infected potato plants in El-Sharkia, Egypt was identified by biological, molecular and serological assays. The aim of this study to predict of antigenic determinants (epitopes) for isolated PVY using immunoinformatics, to prepare antibodies then apply it for PVY screening in potato plants in the field as well as compaired with antibodies against coat protein . The amino acid sequence of PVY isolate was expected by DNAStar protean system in which four parameters for epitope prediction including antigenicity, surface probability, hydrophobicity and secondary structure. These models were applied to PVY as a case study to predict immunogenic peptides for antibodies production. The most hydrophilic regions of PVY-CP were 36-40, 46-47, 67-74, 96-98, 130-133, 164-166 and 198-200 got high score which make it one of the best candidates to be immunogenic and capable of producing antibodies that cross react with PVY. The peptide was chemically synthesized and injected into rabbit. The obtained antibodies were evaluated by using TAS-ELISA and immune dot-blot assay. The obtained antibodies were positively reacted against PVY infected potato tissues.
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