PJB-2018-120
SIMULATANEOUS ANALASIS OF EIGHT STEROIDER SAPONINS DIFFERENT PROCESSED DIOSCOREA ZINGIBERRNSIS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
TING YE
Abstract
Eight major steroidal saponins (parvifloside, protodeltonin, protodioscin, protobioside, zingeberensis newsaponin, deltonin, dioscin, prosaponin A of dioscin) were simultaneously analyzed by HPLC for the quality control of Dioscorea zingiberensis and some other medicinal plants. The reproducibility, stability, precision, recovery, and linear range were studied and showed good precision, reproducibility and linear regression (r2 > 0.9991) within test ranges. Moreover, the changes of eight steroidal saponins content of D. zingiberensis rhizomes with different treatments (wine-processing, hot-air drying, sun-air drying) were investigated. The HPLC method could be applied to simultaneously quantify the eight steroidal saponins in D. zingiberensis. Wine-processing technology could be used to kill the enzymes in D. zingiberensis for gaining more furostan-type saponins with better solubility in water. Hot-air drying technology at 60ºC could be used to gain more spirostan-type saponins. These results might be helpful for D. zingiberensis clinical reasonable application and further studies on the steroidal saponins conversion mechanism.
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