PJB-2018-126
EVALUATION OF ANTIMICROBIAL ACTIVITY OF EX VITRO AND CALLUS EXTRACTS FROM COMMIPHORA GILEADENSIS
OMAR M. AL ZOUBI
Abstract
Commiphora gileadensis a medicinal plant largely used in many Mediterranean and Red sea countries. A large amount of plant materials were needed to produce secondary metabolite under in vitro culture. Therefore, callus is used in the in vitro culture, since it can proliferate quickly and continuously. In the present study, A new and rapid protocol for optimum callus production has been assessed, to overcome limitations of the conventional propagation methods. The effect of plant growth regulator (PGR) on the regeneration of C. gileadensis was investigated for callus induction using a standard MS medium with various concentrations 0.0, 0.5, 1.0,1.5, 2.0 and 2.5 mg/L. The result showed that the maximum regeneration of callus induced the fresh and dry weight were obtained 5675 ± 1321 and 376.7 ± 56.9 mg, respectively on MS media containing 2 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D)+ 0.5 mg/L 6-Benzyl adenine (BA) after 12 weeks. The antibacterial and antifungal activities of C. gileadensis were evaluated using the callus and ex vitro extracts, six bacterial species fungal genera were used the agar well diffusion method. used of 25, 50, 75 and 100 µl methanolic or ethonlic extracts of ex vitro and callus had considerable inhibition effects on the tested bacteria and fungi. Callus culture technique may be an important tool to get the C. gileadensis quickly as compared to the natural growth phenomenon where it takes many years. Moreover, it’s give us an opportunity to get the active constituent without destroying the plant available in nature.
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