PJB-2018-160
TRANSCRIPTIONAL PROFILING AND IDENTIFICATION OF HEAT RESPONSE GENES OF PINEAPPLE FRUITS AFFECTED BY ELEVATED POSTHARVEST TEMPERATURE
CHUAN-HE LIU
Abstract
Elevated postharvest temperature (EPT) stimulated the coloration, and improved the physicochemical quality and the production of aroma compounds of winter-harvested pineapple fruits. However, the metabolic pathways underlying EPT process and the gene expression profiling of the heat response genes as affected by EPT process are not well understood. Here, based on RNA-seq and gene expression profiling, we explored the differentially expressed heat shock protein genes, transcription factor genes and antioxidant enzyme genes of pineapple fruits as affected by EPT at 1 d and 2 d. And, the associated metabolic pathways involved in the EPT process for pineapple fruits were investigated. Illumina sequencing generated 91,829,674 high-quality reads and these were assembled into 61,065 unigenes. DEGs were isolated and annotated to GO classifications. KEGG pathways analysis indicated that DEGs were predominantly enriched to metabolic pathways, biosynthesis of secondary metabolites, and starch and sucrose metabolism. Of the identified DEGs, nineteen HSPs and sHSPs were screened, and most of them were up-regulated by EPT. Most of the transcription factor genes, including HSF, bHLH, WRKY, MYB, AP2/ERF, bZIP and NAC, were down-regulated by EPT. The isolated SOD genes were up-regulated by EPT, while most of the POD and CAT genes were down-regulated. The findings of this work would contribute to understand the molecular mechanisms underlying EPT process, especially the cross-talks among HSPs, transcription factor genes and antioxidant enzyme genes involved in this process.
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