PJB-2018-172
Transcriptome analysis reveals potential genes involved in red pigment biosynthesis in a red pigment radish cultivar (Raphanus sativus L.)
Changman Li
Abstract
Among radish (Raphanus sativus L.) groups, the red pigment radish cultivar (red skin and red flesh) is characterized by its high red pigment content. However, little is known about the molecular mechanisms of the red pigment formation in this special group. Here, by characterizing the transcriptomes of red pigment radish cultivar and those of other two radish cultivars with different colors, we investigated the genetic basis of red pigment biosynthesis in this group. Our gene expression analysis showed that in comparison with other two radish cultivars, 1634 significantly up-regulated genes in red pigment radish cultivar were identified. Among these up-regulated genes, several anthocyanin structural genes were found, such as chalcone isomerase (CHI), chalcone-isomerase 1 (CHI-1), flavanone 3-hydroxylase (F3H), dihydroflavonols 4-reductase (DFR), flavonol synthase 3 (FLS3) and anthocyanidin synthase (ANS). These genes were mainly involved in pigment biosynthetic process, flavonoid biosynthetic process and anthocyanin biosynthetic process. Additionally, our molecular evolution analysis identified 191 putative fast evolving genes in red pigment radish cultivar, these 191 genes may regulate the anthocyanin synthesis processes by influencing expression levels of the anthocyanin structural genes. Overall, our study may provide valuable genetic resources for future studies on radish group and deepen our understandings of the molecular mechanisms of the red pigments biosynthesis in red pigment radish cultivar group.
To Cite this article:
Download
