PJB-2018-177
Management of root rotting fungi and root knot nematode with endophytic fluorescent Pseudomonas associated with Salvadora species
Farzana Korejo, Syed Abid Ali, Fozia Humayun, Afshan Rahman, Viqar Sultana, Jehan Ara and Syed Ehteshamul-Haque
Abstract
Root diseases caused by soilborne plant pathogens are responsible for the losses of billions of dollars in agricultural crops annually. The biological control of soilborne pathogens with microbial antagonists is gaining popularity in the crop protection system, due to the adverse effects of chemicals. In this study 40 isolates of fluorescent Pseudomonas were isolated from roots, shoots and leaves of Salvadora persica L. and S. oleoides Decne. Most of them showed strong suppressive effect on root rotting fungi Macrophomina phaseolina, Rhizoctonia solani, Fusarium solani and F. oxysporum In vitro. Significant nematicidal activity against root knot nematode (Meloidogyne javanica) was also observed by the cell-free culture filtrates of these isolates. Identification of six potential isolates was confirmed by using molecular biology tools. Out of which 3 were identified as Pseudomonas aeruginosa, one each as P. putida and P. monteilii, while one appeared as uncultured Pseudomonas. Experiments conducted on sunflower both in clay pots and field plots, test isolates of endophytic Pseudomonas were found effective in suppressing the root rot disease with improved plant growth as compared to untreated control. Efficacy of some isolates was found comparable with commercial fungicide carbendazim. Some isolates were also found effective in reducing the infestation of root knot nematode under field condition with improved flower weight
To Cite this article:
Korejo, F., S.A. Ali, F. Humayun, A. Rahman, V. Sultana, J. Ara and S.E. Haque. 2019. Management of root rotting fungi and root knot nematode with endophytic fluorescent Pseudomonas associated with Salvadora species. Pak. J. Bot., 51(4): DOI: http://dx.doi.org/10.30848/PJB2019-4(19)
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