PJB-2018-208
Effects of DNA methylation on growth and development of Rehmannia glutinosa
Hongying Duan
Abstract
In this study, 5mC content and methylation level increased along with the growth of Rehmannia glutinosa, but the degree of genomic methylation was different in leaf and root, and, the hemi-methylation level was both higher than the full-methylation level in leaf or root, which was very significant in root. Furthermore, 5-azaC could inhibit genomic methylation of Rehmannia glutinosa, compared with the control, 5mC content was less and showed a decreasing trend along with the increase of 5-azaC concentration, still increased along with the growth of Rehmannia glutinosa and reached the peak in IV growth stage. In addition, as treated with 5-azaC, genomic methylation in Rehmannia glutinosa had significant polymorphism, methylation status would change and appear significant demethylation, and the degree of DNA demethylation increased along with the increasing concentration of 5-azaC. Further analysis found, under the treatment of 5-azaC, plant height and leaf length were both lower than the control, still increased along with the growth of Rehmannia glutinosa, however the phenotype of Rehmannia glutinosa could not appear significant change along with the increase of 5-azaC concentration. Thus, under the reduction of DNA methylation, Rehmannia glutinosa appeared obvious dwarfing phenomenon with smaller leaf, indicating moderate demethylation could inhibit the growth and development of Rehmannia glutinosa, which might be closely related to the status of genomic methylation.
To Cite this article:
Download
