PJB-2018-1638
Transcriptome analysis of Schisandra sphenanthera discovers putative lignan biosynthesis genes and genetic markers
Sen Wang, Zhen Zhang, Wan-Ling Song, Ni-Hao Jiang, Feng-Xia Shao, Sheng-Chao Yang, Fang-De lv, Guang-Hui Zhang, Chun-Hua Ma and Jun-Wen Chen
Abstract
Based on the transcriptional analysis of Schisandra sphenanthera Rehd. Et Wils., a total of 129,951 assembled unigenes were obtained. This article found some cytochrome P450 enzymes (CYP450), such as CYP81Q1 (piperitol/sesamin synthase, PSS), CYP719A24 and CYP71923 catalyze the formation of two methylenedioxy bridges in sesamin and podophyllotoxin biosynthesis, respectively.
The candidates of these CYP450s and O-methyltransferase (OMT) genes that catalyze lignanO-methylation have also discovered from the transcriptome database of S. sphenanthera, indicating that dibenzocyclooctadiene-type lignan might be biosynthesized in the similar pathways of sesamin and podophyllotoxin. Moreover, cloning and functional characterization of those genes will help us to illuminate the biosynthesis mechanisms of dibenzocyclooctadiene-type lignan. Additionally, twenty sets of primers for SSR were chosen in random for validating the polymorphism and amplification. The results showed that 18 (90.00%) primer pairs could be amplified successfully and 15 (83.33%) primer pairs exhibited polymorphisms.This study represents the first report to analyze the transcriptome of S. sphenanthera using high-throughput RNA-seq technology. These data will enrich the genomic data and provide a solid evidence for functional genomics and molecular genetic researching in this herb.
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