PJB-2018-238
PHYTOCHEMICAL APPRAISAL AND BIOLOGICAL EVALUATION OF TRADITIONAL FOOD PLANT CARALLUMA TUBERCULATA
Muhammad Waleed Baig
Abstract
Food plants possess both nutritional and medicinal properties. We assessed Caralluma tuberculata plant extracts for total phenolics content, total flavonoids content and HPLC-DAD. In addition, in-vitro antioxidants, antimicrobial, cytotoxic, protein kinase inhibition and ex-vivo spasmogenic/spasmolytic assays were conducted. Maximum phenolics and flavonoids contents were computed in distilled water extract i.e. 16.21±0.5 μg gallic acid equivalent/mg extract and 7.56±0.4 μg quercetin equivalent/mg extract. HPLC-DAD quantified rutin (0.581 µg/mg extract) and gallic acid (0.35 µg/mg extract) in methanol-ethyl acetate and methanol extracts respectively. Water-acetone extract exhibited highest DPPH scavenging (36.13±0.84%), total antioxidant (92.21±0.7 μg ascorbic acid equivalent/mg extract) and total reducing potential (76.01±0.9 μg ascorbic acid equivalent/mg extract). Maximal antifungal effect against Mucor spp. was observed to be MIC: 50 µg/disc using ethyl acetate-methanol extract, antileishmanial (IC50: 120.8±3.7 µg/ml; n-hexane extract), brine shrimp cytotoxicity (LD50: 29.94±1.6 µg/ml; ethyl acetate extract), THP-1 cytotoxicity (IC50: 118±3.4 µg/ml; distilled water-acetone extract) and protein kinase inhibition (ZOI: 19±1 mm; methanol-chloroform extract). Methanol extract of Caralluma tuberculata exerted dose dependent spasmolysis (3.0-10.0 mg/ml) in rabbit jejunal tissue in response to spontaneous and K+ (80 mM) induced contractions comparable to verapamil as well as spasmogenic (0.03-3 mg/ml) effect, which was reversed by pretreatment with atropine. On tracheal tissue, it produced relaxation of K+ and carbachol (1 µM) induced contractions. Plant extracts exhibited muscarinic, anti-muscarinic and calcium antagonist potentials. Our findings suggest that C. tuberculata is a potent therapeutic candidate with profound biological aptitude. Keywords: food plant; Caralluma; spasmolytic; cytotoxicity; antioxidant
To Cite this article:
Download
