PJB-2018-241
EVALUATION OF WILD RHYNCHOSIA CAPITATA (ROTH, DC) THROUGH MORPHOLOGICAL AND BIOCHEMICAL MARKERS, COLLECTED FROM THE HILLY AREAS OF DISTRICT DIR (LOWER), KHYBER PAKHTUNKHWA, PAKISTAN
MURAD ALI
Abstract
Legumes play a vital-role in natural ecosystems, agriculture and agroforestry, but the yields unfortunately continues to lag behind those of cereals and needs extensive explorations, collection and evolution through markers assisted selection to meet the market challenges. In this study we explored the wild Rhynchosia capitata (Roth, DC.) through morphological and biochemical markers. For the first time we reported eight (leaf margin, leaf colour, flower colour, pod shape, seed shape testa colour, seed spot and seed colour) qualitative (fig.1) and 11 (leaf length, leaf width, number of branches, petiole length, pods length, pod width, seed length, seed width, seed thickness, 100-seed weight, total biomass) quantitative characters were recorded. The passport information for the ethno botanical uses of genotypes only in one location (pashatai) out of 10/50 location it is used for stomach disorder according to 10% people. The forage usage in 10 locations out of 50 ranging from 5% to 30% in each location. While in unknown include green manure according to 10% people, 15% help in nitrogen fixation, 20% birds eating their seed and 20% due to which poor growth of mung respectively according to people opinion (table.1). Circumstance of measurable characters, major variation was observed for all characters except number of branch per plant, 100 kernel weight, flower colour and seed spot for which low difference was detected. The outcomes of statistical analysis shows important changes in all calculated characters (Table. 2), like maximum coefficient of variation (CV) shown by petiole length (50.97%) and minimum by seed thickness (6.84%). Seed protein summary shows that differences in main bands were present in landraces RH006, RH007, RH014, RH017, RHO26, RH027 and RH028, whereas significant variations were present in minor bands in the accession RH006, RH007, RH009, RH014, RH015, RH016, RH017, RH022, RH024, RH025, RH026, RH027 and RH028. Considerable variations were also recorded in density of some common bands. Cluster analysis revealed significant genetic diversity which is 0.58% in band 2 and 0.2% in band 14 from banding profile. It is due to Geographical association created on whole seed protein outlines, similar germplasm in diverse zones and negative effect of genetic factor in diverse lines. Key words: Rhynchosia capitata L., Genetic divergence, Morphometric, SDS-PAGE, Statistical analysis
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