PJB-2018-304
PSEUDOMONAS OTITIDIS AND BACILLUS SUBTILIS FROM SALINE SOIL OF PAKISTAN SHOWED A POTENTIAL OF CALCIUM PRECIPITATION
AISHA WAHEED QURASHI
Abstract
Biomineralisation is an important phenomenon reported in microbes of different environments. Present study deals with the analysis of mineralization potential of two salt tolerant species of IQ (Pseudomonas otitidis) and NAY (Bacillus subtilis) isolated from rhizosphere of plant. These strains have the ability to show precipitation in acetate supplemented medium for 21 days of culture incubation and showed positive results for urease tests. Increase in microbial precipitation was recorded in both strains however, strain NAY (Bacillus subtilis) showed more precipitation as compared to other IQ (Pseudomonas otitidis). It was also observed that this precipitation was associated with increase in pH of the medium from 7.0 to 8.0 that started to increase at 4th day of culture incubation and increased slightly till 15 days. The FTIR analysis of the calcite crystals showed visible peaks at 711-1400 cm-1 wavelength. The visible bubbling appeared in acid test assay further confirmed the calcite precipitation. The mineral precipitation in both species under salt stress is significant for soil consolidation as well as adjusting soil pH under acidic conditions.
To Cite this article:
Download
