PJB-2018-311
DEVELOPMENT AND APPLICATION OF A SINGLE NUCLEOTIDE POILMORPHISM MARKER FOR NtCPS2, A KEY GENE CONTROLLING CIS-ABIENOL SYNTHESIS IN TOBACCO GLANDULAR TRICHOMES
aixia,chang;guoping,wang
Abstract
Cis-abienol, an important compound responsible for flavor and aroma characteristics of tobacco, is a major labdanoid diterpene secreted from trichomes of most oriental tobacco and some cigar tobacco varieties. Developing molecular markers is one of the most effective ways for transferring this aroma trait to flue-cured tobacco. Based on the technique of allele-specific PCR (AS-PCR), in the present study, we developed two polymorphic primer pairs and verified them using six parents and the F1 population. Genotyping of flue-cured tobacco plants and high aroma mutants of Zhongyan100 with the two primer pairs identified the cis-abienol containing Dabaijin599, Gexin3, NC2326, varieties and one mutant line, TBT6. The results indicated that these primers can be used for molecular marker-assisted selection in tobacco breeding. The flue-cured germplasms containing cis-abienol can be useful as parents in tobacco breeding.
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