PJB-2018-315
AFLP analysis of resistance to Colletotrichum gloeosporioides in Camellia oleifera (Theaceae)
Zhihua Cao, Cuiping Cao, Juanjuan Hu, Qinglong Shu and Reiner Finkeldey
Abstract
Anthracnose is a highly destructive fungal disease caused by Colletotrichum gloeosporioides that results in severe economic losses to Camellia oleifera production. Herein, we investigated whether genotypes identified as resistant or susceptible to anthracnose in China could be distinguished using amplified fragment length polymorphism (AFLP) markers. A total of 30 unrelated C. oleifera genotypes were selected from three ecotype regions (Huangshan, Shucheng and Fengyang) in Anhui Province. Resistance was assessed by disease severity in plants following inoculation of detached fruit. AFLP selective primer combinations were used to identify C. oleifera genotypes. We amplified 147 bands, of which 129 (87.76%) were polymorphic and used to analyse genetic diversity. Among resistant C. oleifera, genetic similarity coefficients ranged from 0.40 to 0.85, indicating high genetic diversity. The 30 genotypes grouped into two major clusters based on polymorphic bands. This study provides knowledge of genetic diversity that will be useful for future breeding of C. oleifera for anthracnose resistance.
To Cite this article:
Cao, Z., C. Cao, J. Hu, Q. Shu and R. Finkeldey. 2019. AFLP analysis of resistance to Colletotrichum gloeosporioides in Camellia oleifera (Theaceae). Pak. J. Bot., 51(6): DOI: http://dx.doi.org/10.30848/PJB2019-6(14)
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