PJB-2018-379
PHYTOCHEMICALS, ANTIBACTERIAL AND ANTIOXIDANT INVESTIGATIONS FROM SEED EXTRACTS OF PROTORHUS LONGIFOLIA (BERNH. EX C. KRAUSS) ENGL.
Amanda Perumal
Abstract
Protorhus longifolia (Anacardiaceae) is a popular medicinal plant in South Africa and is commonly used in ethnomedicine to treat a variety of ailments. In this study, we attempted to identify the possible antimicrobial, antioxidant and anticancer properties of different crude extracts from seed. Preliminary phytochemical screening showed the presence of cardiac glycosides, phenols, sterols and flavonoids in the plant extracts with phytocompounds of pharmacological importance identified via Gas Chromatography-Mass Spectroscopy (GC-MS) analysis. The methanol and ethanol extracts exhibited antioxidant potential with IC50 of 5.00 ± 0.33 µg/mL and 32.61 ± 0.42 µg/mL respectively. The antibacterial activity of the seed extracts was determined using disc diffusion method. All seed extracts exhibited an antibacterial effect against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. Ethanol crude extract displayed minimum inhibitory concentration (MIC) of 98.61 µg/mL against Gram-negative bacteria P. aeruginosa. Interestingly, no antifungal activity was noted from crude extracts of seed from P longifolia. The anti-proliferative activity of the methanol and ethanol extracts against MCF-7 cells was within the limits (IC50 less than 30 µg/mL) stipulated by the American National Cancer Institute (NCI) for cytotoxicity. These results endorse the potential of seeds from P. longifolia for the search of new antimicrobial and anticancer drugs and a source of antioxidants.
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