PJB-2018-395
ALLELOPATHIC EVALUATION OF PINUS GERARDIANA USING ORGANIC FRACTION GUIDED BIOASSAY AND ITS PHYTOCHEMICAL SCREENING
Dr. Zahir Muhammad
Abstract
Screening of phytoconstituents of a particular plant and subsequent assessment of its extracts on test plants helps in identification of allelopathic properties of the plant in question. This would lead proper cultivation managements of other crops with respect to allelopathic plant. In this study, we determined phytoconstituents of Pinus gerardiana and evaluated its allelopathic activities on wheat. The ethanolic extract bioassay of leaves, root, bark and their fractions i.e. iso-butanol, water, ethyl acetate and n-hexane fractions were conducted on wheat (cv Siran). Germination, growth and biomass parameter were observed under the allelopathic study whereas under phytochemical study, qualitative screening of the phytoconstituents was also observed. In the ethanolic crude extract of leaf, root and bark of P. gerardiana germination were completely absent. However, according to fraction bioassay leaf fraction shows maximum inhibition as compared to root and bark fractions. The organic fraction iso-butanol imparted maximum inhibition effect on germination, growth and biomass parameter of wheat. Phytochemical investigation showed the presence of carbohydrate, phenol, phytosterol, terpenoids, steroids, quinones and saponin in the crude extracts of leaf, root and stem bark of P. gerardiana. Interestingly, iso-butanol organic fractions were found more toxic than the other fractions while leaves exhibited stronger allelopathic activity than root and barks. The study confirms that P. gerardiana is a potent allelopathic plants which contains diverse chemical compounds.
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