PJB-2018-418
Identification, analysis and evaluation of starch branching enzyme gene promoter from Oryza sativa
Pakeeza Rubab, Hira Mubeen, Ammara Masood, Javaid Iqbal Wattoo and Mushtaq A. Saleem
Abstract
Promoters are regulatory elements that control’s transcription and overall expression profile of genes. Plants consists of several enzymes, which are important for controlling metabolic activities. Starch branching enzyme (SBE), is one of an important enzyme involved in biosynthesis of starch in plants. It also plays a crucial role in determining the structure and physical properties of starch granules. SBE has two types, SBEl and SBEll. SBEll further divided in SBElla and SBEllb. Both of these have different effects and size in different crops. Starch branching enzyme from Oryza sativa was selected for the current research. Rice is a staple food of 70% population of the world. The present research was focused on identification of SBE gene orthologues, analysis of SBE promoter sequence through High throughput genome sequencing (HTGS) database, screening of cis regulatory elements through Plant CARE database and further detection of putative protein domains through Conserved protein domain family (CDD) database within the promoter region. Several bioinformatics software’s were used for this purpose. The study provides a deep insight on importance of designing constitutive novel promoters, which can be effectively substituted to get enhanced transgene expression in agricultural crops
To Cite this article:
Rubab, P., H. Mubeen, A. Masood, J.I. Wattoo and M.A. Saleem. 2019. Identification, analysis and evaluation of starch branching enzyme gene promoter from Oryza sativa. Pak. J. Bot., 51(6): DOI: http://dx.doi.org/10.30848/PJB2019-6(15)
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