PJB-2018-440
PHYTOCHEMICAL, ANTIMICROBIAL AND CYTOTOXIC ACTIVITIES OF GAULTHERIA TRICHOPHYLLA ROYL
Shah Zaman
Abstract
ABSTRACT:
Context: Gaultheria trichophylla fruit is used to treat asthma, headache and as an appetizer of alpine and sub-alpine region of Western Himalaya. Species of Gaultheria trichophylla Royle. and its align have their restricted distribution to Western Himalayas. This plant is broadly used by indigenous people; however, no studies found proving its antimicrobial significance.
Objective: This study determines antimicrobial activities of Gaultheria trichophylla extracts and subsequent fractions. The study is based on the folk uses of the medicinal plant in traditional communities.
Materials and methods: In the current manuscript we demonstrate the phytochemical tests, in vitro cytotoxic and antimicrobial effects of different fractions of G. trichophylla. Four (4) different bacteria (Escherichia coli, Bacilus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus) as well as 4 fungal strains (Helminthisporium aereus, Fusarium solani, Aspergilu sflavus and Aspergilus fumigates) are characterized in the screening of extract.
Results: Qualitative phytochemical screening showed the existence of different active compounds. Quantitative phytochemical screening showed the existence of phenolics ranging from 3.27±0.44 mg/g to 14.17.9±088.mg/g, flavonoids from 8.08±0.48 mg/g to 26.9±0.23 mg/g. The cytotoxic assay showed LC50 of 50 μg/ml for methanolic extracts. Antifungal assay of methanolic and subsequent fractions against different fungal strains showed MIC ranging from 29±1.154% to 86.66 ±0.09% and 7.5mg/ml to 15mg/ml MIC value for antibacterial assay.
Discussion and Conclusion: Phytochemical screening provides a tool for active ingredients. Methanolic and subsequent fractions of Gaultheria trichophylla reveal most excellent activities in each assay, in cytotoxic activities and in inhibition of microbes. The potent of activities is because of the existence of bioactive phenolics, flavonoids, and saponins compounds.
Keywords: Gaultheria trichophylla, Phytochemical, cytotoxic and antimicrobial activity.
ABBRIVIATIONS: GTME =Gaultheria trichophylla methanolic extract, GTHE = N-hexane extract, GTCE = Chloroform extract, GTEE = Ethyl acetate extract, GTBE = Butanol extract, GTWE = Water extract.
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