PJB-2018-454
GENETIC DIVERSITY AND POPULATION STRUCTURE OF QUINOA (Chenopodium quinoa WILLD.) GERMPLASM CULTIVATED IN TURKEY BY USING iPBS MARKERS
Arash Hossein Pour
Abstract
Quinoa (Chenopodium quinoa Willd.) is nutritionaly effective plant by having a good protein quality as well as a large number of vitamins and minerals. It has been cultured for several thousand years in South America,. We investigated the utility of inter-primer binding site (iPBS) for the molecular characterization of 17 Quinoas (Chenopodium quinoa Willd.) cultivated in Turkey. Here, 25 iPBS markers were employed, but 6 primers gave sufficient polymorphic data generating a total of 19 alleles with an average of 2.83 bands/ primer. The amount of iPBS bands per individual was calculated as 1.12. The PIC (Polymorphism Information Content) rate ranged from 0.02 to 0.49, with an average of 0.20. Genetic associations assessed by using the Dice dissimilarity coefficient generated values between different pairs of accessions that with an average of 0.84 for France population and Q-52 genotypes. Cluster analysis conferring to the unweighted pair-group mean average (UPGMA) divided 17 Quinoas genotypes into two major clusters. Outcome of principal component analysis (PCA) was in agreement with cluster analysis. The highest number of alleles (ne), Nei’s genetic diversity (h) and Shannon’s information index (I) were obtained from French Vanilla genotype at 1.99, 0.50 and 0.69, respectively, whereas the lowest values were observed in Q–52 genotype at 1.10, 0.09 and 0.20, respectively. The expected heterozygosity fluctuated from 0.398 in the first sub-population to 0.140 in the second sub-population with an average of 0.269. The mean population differentiation measurements (Fst) values of the sub-populations were 0.048 and 0.676. The outcomes will give a useful information for the management of germplasm and contribute to improvement of existing breeding approaches. The results also presented that the iPBS marker system is a suitable tool for identification and genetic diversity analysis of Quinoas genotypes.
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