PJB-2018-495
Responses of potato plantlets cultured in virto under fluorescent and light-emitting diode (LED) source
Lili Jiang
Abstract
The objective of this study was to determine the effects of different light-emitting diode (LED) light sources on the growth of potato (Solanum tuberosum L.) plantlets. Shoot cuttings of potato cultivar ‘ Favorita’ were transplanted on MS medium and cultured for 21 days in vitro under six different lights qualities: fluorescent lamp (FL), 100% red LED (100R), 100% blue LED (100B), three blue and red LED mixtures (80R/20B, 70R/30B, 50R/50B). The effects of the six light sources on growth, morphogenesis and tuberization of potato plantlets grown in vitro were investigated. Plant height was greatest in plantlets cultured under 100R LED, Root lengh, Fresh weight were greatest in plantlets cultured under 80R/20B LED, chlorophyll content, carotenoid content, number of tuber per plant, diameter of the biggest tuber were greatest in plantlets cultured under 70R/30B LED, average weight of single tuber, total soluble carbohydrate content were greatest in plantlets cultured under 50R/50B, and they were lower in plantlets cultured under a fluorescent light. The principal component analysis revealed that the first 2 components with eigen value of greater than 2.175 contributed about 91.016% of variation. This implied that these traits should be given emphasis for evaluating LED treatment. According to principal components value of 6 LED treatment, 70R/30B LED was the most suitable light for the growth of upland cotton plantlets in vitro.
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