PJB-2018-512
Isolation and characterization of the lipoxygenase-3 promoter in Nicotiana tabacum
Minmin Xie, Shanshan Li, Zhiyuan Li, Mingli Chen, Daping Gong and Aurore Richel
Abstract
The jasmonates (JA) play key roles in defending mechanical wounding, herbivore attack and various stresses in plants. Lipoxygenase (LOX) is one of the key enzymes in the biosynthesis of JA which also catalyzes the green leafy volatiles synthesis at the first step. Wounding and herbivore attack can induce the expression of LOXs. To investigate the molecular mechanism regulating the LOX genes in tobacco, we isolated and identified the LOX3 gene promoter from Nicotiana tabacum. Analysis from transgenic plants and GUS activity fluorimetric assay revealed that 1847bp of NtLOX3 promoter sequence was sufficient for wounding and methyl jasmonic acid (MeJA) induction. For wounding and MeJA induction, the deletion analysis identified the minimal effective regions which were located in -546bp to -199bp. And this region contained the cis-acting elements of CATG-box (CATG), W-box (C/TTGACY) and a T/G-box (AACGTG), which were thought to play a key role in wounding and MeJA elicitation. These results may provide information for further transcriptional regulation studies of LOX genes.
To Cite this article:
Xie, M., S. Li, Z. Li, M. Chen, D. Gong and A. Richel. 2024. Isolation and characterization of the lipoxygenase-3 promoter in Nicotiana tabacum. Pak. J. Bot., 56(1): DOI: http://dx.doi.org/10.30848/PJB2024-1(6)
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