PJB-2018-546
Production of cellulase and xylanase from Candida tropicalis (MK-118) on purified and crude substrates
Maria Shariq and Muhammad Sohail
Abstract
Lignocellulose, a mixture of cellulose, hemicellulose and lignin, is naturally occurring chemical feedstock on earth which can serve as substrate for the production of plant cell wall degrading enzymes (PCWDE) including cellulase and xylanase. Yeast strains have scarcely been reported for the simultaneous production of more than one PCWDEs. In this study, an indigenous strain of Candida tropicalis MK-118 has been reported for the potential to co-produce endoglucanase (EG), β-glucosidase (BGL) and xylanase (Xyl). It was observed that the production of an individual enzyme varied differently with a change in cultivation conditions. For EG and BGL production, cultivation at 40oC was more suitable while the highest titers of Xyl were obtained at 25oC. Higher yields of EG and Xyl were obtained under neutral pH, whereas, acidic medium favored BGL production. Although, an inoculum size of 2% was found appropriate for all the three enzymes, however, maximum production of EG, BGL and Xyl was obtained in presence of 0.5% carboxymethyl cellulose, 1.5% salicin and 1% xylan. Furthermore, the enzymes were characterized for their optimal activities. The strain showed ability to ferment sugarcane-baggase and wheat-bran with the concomitant production of cellulases and xylanases. Interestingly, the strain also produced alcohol in complex medium. C. tropicalis MK-118 may find its application for the production of industrially important enzymes and alcohol under submerged fermentation of crude substrates. The properties of enzyme preparation were industrially relevant and hence the enzyme can also be applied for various processes
To Cite this article:
Shariq, M. and M. Sohail. 2020. Production of cellulase and xylanase from Candida tropicalis (MK-118) on purified and crude substrates. Pak. J. Bot., 52(1): DOI: http://dx.doi.org/10.30848/PJB2020-1(14)
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