PJB-2018-1777
Identification of GS1a, GS1b and GS1c genes from Eichhornia crassipes and their transcript analysis in response to different nitrogen sources
Minghui Fu, Lihua Jiang and Guohua Yan
Abstract
Eichhornia crassipes was one of the fastest growing plants on earth. It can assimilate nitrogen very efficiently. Glutamine synthetase (GS) is a key enzyme in nitrogen metabolism. Thus research of GS in Eichhornia crassipes could help to explain the mechanism of the ability of E. crassipes to assimilate nitrogen. In this study, we cloned three cytosolic GS cDNAs, GS1a, GS1b and GS1c from the roots of E. crassipes using the RACE method, studied the characterizations with the yeast complementation experiment and analyzed the expressions of GS1a, GS1b and GS1c with qRT-PCT in response to different nitrogen sources in roots and leaves. They were approximately 1400-1500 nucleotide encoding proteins with 354-356 amino acids that functionally complemented the mutant yeast ΔGln for growth on ammonium as the sole source of nitrogen. Their relative expression level was markedly up-regulated under the status of nitrate as the only nitrogen source except that of GS1b in roots, which was only up-regulated less than one fold, and GS1a in leaves, which was up-regulated under condition of nitrogen deprivation. All these results suggested that E. crassipes has at least three different cytosolic GS1 genes which have typical conserved domains of GS gene and can functionally complement the mutant yeast that is deprived of the GS gene. All these E. crassipes GS1 genes have different expression patterns in response to different nitrogen sources.
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