PJB-2018-1779
Genetic mapping and QTL analysis associated with fusarium head blight resistance at different developmental stages in wheat (Triticum aestivum L.) using recombinant inbred lines
Le Chang, Zhiyuan Guo, Ximei Li and Ping Mu
Abstract
In order to reveal the genetic basis of wheat FHB resistance of a recombinant inbreed line (RIL) population including 160 plants derived from a cross between PI277012 and Yumai 18 was used in this study. Wheat recombinant inbred line (RIL) population was inoculated in a suspension of Fusarium graminearum spores and cultured indoor, during which pathogenesis was observed. Disease index and area under the disease progress curve (AUDPC) were calculated based on the data that collected on days 5, 10, and 15, in order to analyze the dynamic changes in wheat FHB resistance at different developmental stages. During the first stage, no QTL (quantitative trait locus) was found, and disease resistance index of each strain was increased steadily and uniformly. We speculated that when Fusarium graminearum started to expand in wheat spikelet, the wheat plant has yet to respond to the infection, resulting in a minor difference in disease resistance between the different lines. During the second stage, four QTLs were found, which were mapped to chromosomes1A, 1D, 3B and 4D, respectively, with QTLs located between wmc777 and barc147on chromosome 3B, which was consistent with previous results. We speculated that the large differences in resistance index in this stage were attributed to the reaction of the FHB-resistant QTLs, leading to differences among different wheat strains. During the third stage, three QTLs were located on chromosomes1A, 5A and 7A, respectively. Compared with the traditional field cultured inoculation, field topsoil inoculation or natural breeding, the indoor experiment was highly controlled, facilitating annual comparison independent of variations in climate.
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