PJB-2018-1786
In silico profiling and characterization of conserved microRNAs in biofuel plant sorghum
Muhammad Din, Muhammad Younas Khan Barozai and Ahmad Naseer Aziz
Abstract
MicroRNAs (miRNAs) are a class of small RNAs, noncoding and transcribed endogenously that directly involved in regulating gene expression at the post-transcriptional level. Majority of the miRNAs are highly conserved in plants that provide the rationality for identification of new conserved miRNAs in other species of plants through comparative genomics. In this research project, the reported known plant miRNAs were blasted against the Expressed Sequence Tags (ESTs) database of Sorghum, and following the series of stringent criteria of miRNAs profiling and characterization via homology search. Hence, a total of 25 miRNAs were identified in biofuel plant Sorghum. For them 4247 potential target genes were also predicted which belongs to the 142 Gene Ontology (GO) enrichment terms of biological processes, molecular functions and cellular components. These GO enrichment terms appear to be involved in transcription factors, growth and development, metabolism, stress related, disease related and other physiological processes. The findings of this research will enable the researchers for better devising of Sorghum plant for biofuel qualities and traits.
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