PJB-2018-1811
A biosynthesis method to produce resveratrol in Saccharomyces cerevisiae with secretion expression of grape anti-oxidant resveratrol synthase gene during wine fermentation
Yangbo Song, Li Feng, Dongqing Ye, Yuyang Song, Yi Qin, Yanlin Liu and Shuwen Liu
Abstract
Resveratrol (Res), a stilbenic compound, recently has become the focus of a number of studies in medicine and plant physiology, and Saccharomyces cerevisiae (S. cerevisiae) has become a popular host for production of non-native compounds. As the last and key enzyme of the biosynthesis pathway, the amount and expression level of Resveratrol Synthase (RS) positive correlation on the production of Res. In this study, a signal peptide sequence, secreted by α-factor of S. cerevisiae, was connected with the RS gene, resulting a pYCα secretory plasmid construction. The RS gene was secretory expressed in S. cerevisiae successfully. Subsequently, a white wine fermentation was carried out by the recombinant strain. The Res concentration of the white wine fermented by the recombinant strain was 36% higher than the control. This has great interests for establishing a controllable way to produce RS and lays the foundation for exploring the Res biosynthesis method.
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