PJB-2018-567
AN EFFICIENT IN VITRO PROPAGATION PROTOCOL OF DIANTHUS GIGANTEIFORMIS BORBAS SUBSP. KLADOVANUS (DEGEN) SOO
Marija Marković
Abstract
Dianthus giganteiformis subsp. kladovanus is an endemic, endangered perennial plant that can be used as an ornamental, especially in sandy soils. The most suitable method for its propagation and ex situ conservation is micropropagation. For this reason, the experiments were conducted in order to establish an efficient protocol for the micropropagation of this subspecies. The in vitro culture was established from seeds and germination percentage was high (88%). According to the results obtained in this study, the multiplication phase should be performed on a half-strength MS medium containing 0.1 mg L-1 BAP and 0.1 mg L-1 NAA. In vitro rooting was successful on a hormone-free half-strength MS medium (91.7%). The addition of NAA slightly increased rooting percentage (95%). Obtained microplants were successfully acclimatized (83.3%) in a 1:1 mixture of peat and sand. Using the protocol presented in this paper, efficient propagation of D. giganteiformis spp. kladovanus can be achieved for both plant production aimed at biodiversity protection and floricultural production of this species.
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