PJB-2018-570
ANTI-INFLUENZA VIRUS ACTIVITY OF SALVIA MILTIORRHIZA BUNGE AND CONSTITUENTS CHARACTERIZATION
ZHIXIA DU
Abstract
This study aimed to investigate the neuraminidase (NA) inhibitory activity of Salvia miltiorrhiza Bunge and provide new resources for anti-influenza virus drugs. Two S. miltiorrhiza root extracts were evaluated for firstly their anti-influenza virus activity by using influenza virus NA inhibition assay. Active compounds of the extracts were tentatively characterised by ultra-high-performance liquid coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Thirty-six compounds were identified in the ethanol extract by using UPLC-Q-TOF-MS. Eight of the major identified compounds were docked with the NA target, together with their explored NA activity. Results indicated that the etnanol extract presented better NA inhibitory activity than the petroleum ether extract. Moreover, eight constituents, namely, lithospermic acid, rosmarinic acid, tanshinone IIA, salvianolic acid A, salvianolic acid B, dihydrotanshinone, tanshinone I and cryptotanshinone, displayed strong docking effects. Among them, lithospermic acid (IC50=157.44) and rosmarinic acid (IC50=204.74) exhibited the highest NA inhibitory activity, exceeding that of oseltamivir acid (IC50=361.83). Meanwhile, docking results were consistent with the results of the NA inhibitory activity assay. These discoveries show that S. miltiorrhiza exhibits NA inhibitory activity. However, its clinical research should be further explored.
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