PJB-2018-576
ENHANCEMENT OF QAULITY AND YIELD IN NOTOPTERYGIUM FROBESII BY LOWER ALTITUDE CULTIVATION
Yehong Guo
Abstract
In order to explore the influence of lower-altitude cultivation on constituents and yield on the Notopterygium frobesii. In this study, a reverse phase high-performance liquid chromatography (RP-HPLC-DAD) method based on the “Chinese Pharmacopoeia” was established for the determination of Isoimperatorin, notopterol, and ferulic acid the main bioactive components described in Pharmacopoeia. The different altitude areas(Lanzhou-Campus, Tianzhu-Hillside, Tianzhu-Farmhouse) of Qianghuo harvested in different months were determined and the influences of cultivation altitude on the quality and yield of Qianghuo were also analyzed. The results showed that contents of the three constituents of Qianghuo cultivated in Lanzhou(lower-altitude) meet the criteria of the Pharmacopoeia of the People's Republic of China; however, the constituents of Qianghuo cultivated in Tianzhu(higher-altitude) do not meet the standard of the Chinese Pharmacopoeia. The contents of the three constituents in the stems and leaves of Notopterygium frobesii do not accord with the standards set in the Pharmacopoeia. However, alternative utilization of these parts can be considered as upplement for livestock fodders. This study concluded that the 4-year-old Notopterygium frobesii was the highest active ingredient of Qianghuo, and October was the best harvesting period. Qianghuo can be cultivated in lower-altitude farmland at an elevation of 1500 m as well.
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