PJB-2018-583
Expression Pattern of Superoxide Dismutase Genes under Water Deficit stress in Maize
Khosro Mafakheri
Abstract
Climate changes and subsequent water-deficit stress are most important limiting factor for crop production in Iran and all over the world. A field trial was conducted with three levels of water-deficit stress (well-watered, intermediate stress and severe water-deficit stress) as one factor and maize genotypes as other factor using three randomized complete blocks. Two cultivars Sc706 and Sc260 were identified as most susceptible and tolerant genotypes respectively, and the expression of some encoding genes for superoxide dismutase was studied. Mn-SOD, Cu/Zn-SOD, Fe-SOD and 18S rRNA control genes were studied. Data analysis for gene expressions by Real-Time PCR showed that as stress severity increases, gene expression increases. However, gene responses in two genotypes and three irrigations treatment were different. Interactions between genotype and water-deficient stress for Fe-SOD and Mn-SOD encoding genes were significant. The gene expression for Mn-SOD in intermediate stress for Sc260 was significantly higher in comparison to Sc760; there was not significant in severe stress. Gene expression for Fe-SOD in intermediate and severe stress for Sc706 was significantly higher. Interaction between genotype and water-deficit stress for Cu/Zn-SOD was not significant, so that gene expression was increased 37.4 and 195.7% in intermediate and severe stress, respectively. This problem could indicate the higher importance of Cu/Zn-SOD gene in superoxide scavenging and adaptation of maize plant.
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