PJB-2004-62
APPLICATION OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) TECHNIQUE FOR THE IDENTIFICATION OF MARKERS LINKED TO SALINITY TOLERANCE IN WHEAT (TRITICUM AESTIVUM L.)
MEHBOOB-UR-RAHMAN, TANWIR AHMAD MALIK1, MUHAMMAD ASLAM CHOWDHARY1, MUHAMMAD JAVED IQBAL2 AND YUSUF ZAFAR1
Abstract
DNA marker for a low Na uptake trait in hexaploid wheat (Triticum aestivum L.) was identified. The individual plants from F3 population segregating for salinity tolerance and the parents (LU-26S & Rohtas-90) were grown in polyethylene tubes under saline conditions (EC 25 dS m-1) and screened for K:Na ratio, chloride ions and net photosynthesis at the fourth leaf stage. The plants were then transplanted into pots filled with 7 Kg of fertile soil and supplied with optimum water and nutrients until maturity. Correlations of K:Na and net photosynthesis with yield components were calculated. Genomic DNA from 15 tolerant and 15 sensitive F3 plants was extracted. The bulked segregant analysis was used in the random amplified polymorphic DNA (RAPD) technique. DNA polymorphisms were observed using 148 primers. The primer OPZ-10 amplified a 680 bp polymorphic DNA fragment which linked to K:Na ratio trait. This DNA fragment can be used for marker-assisted selection to breed for salinity tolerant wheat. The K:Na ratio and net photosynthesis were not correlated with yield components.
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