PJB-2018-605
IDENTIFICATION OF PHOSPHORYLATION PROTEINS IN RESPONSE TO WATER DEFICIT DURING WHEAT FLAG LEAF AND GRAIN DEVELOPMENT
Fei Luo
Abstract
Protein phosphorylation is one of the most important post translation modifications, widely participating in plant abiotic stress regulation. In this study, we performed the first comparative analysis of phosphorylated protein characterization in flag leaves and developing grains of elite Chinese bread wheat cultivar Zhongmai 175 under water deficit by combining with proteomic approch and Pro-Q Diamond gel staining. Field experiment showed that water deficit caused significant reduction of plant height, tiller number, ear length and grain yield. 2-DE and Pro-Q Diamond gel staining analysis showed that 58 proteins were phosphorylated among 112 differentially accumulated proteins in response to water deficit, including 20 in the flag leaves and 38 in the developing grains. The phosphorylated proteins from flag leaves mainly involved in photosynthesis, carbohydrate metabolism and energy metabolism while those from developing grains mainly participated in detoxification and defense, protein metabolism, carbohydrate metabolism and energy metabolism. Particularly, water deficit resulted in significant downregulation of phosphorylated modification level in the flag leaves, which could affect photosynthesis and grain yield. However, some important phosphorylated proteins involved in stress defense, energy metabolism and starch biosynthesis were upregulated under water deficit, which could benefit drought tolerance, accelerate grain filling and shorten grain developing time. Our results provide new insights into the molecular mechanisms how phosphoproteins respond to drought stress and thus reduce production.
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