PJB-2018-617
Pollen biology of Grewia optiva Drummond genotypes: an important agroforestry tree of North Western Himalayas
Saresh N V
Abstract
Grewia optiva is an important agroforestry tree of Himalayas because of its account of utility as fodder fuel and fibre. Hence determining the components of pollen biology is important for its breeding and conservation. In the present investigation it was observed that pollen grains of Grewia optiva were isopolar radio symmetric normal and fertile and size ranges from 49.00 to 74.37μ. Positive correlation was observed among anther and flower levels (0.985) anther number and pollen production per anther (0.686) and anther number and pollen production per flower (0.800). Pollen viability rates significantly differed among freshly collected pollen grains and stored pollen grains at 4oC and -20°C. The percentage of fruit set were found to decrease towards the collection time of pollen grains at the beginning of anther dehiscence (68-79%) after 6 h (39-53%) and 24 h (26-38%) of anthesis. The present experiment showed that 20% sucrose with BKM (Brewbaker and Kwack’s medium) was the optimum medium for the germination of pollen grains. Germination percentage was maximum in genotype BI-4 (20.40 %) and minimum in SO-3 (5.20 %). The stigma became receptive 14-20 hours after pollination and the species is found to be self-compatible. The variation in size pollen production viability and germination percent was because of genetic differences among the genotypes and external factors like temperature humidity etc. The findings provide important insight to understand the reproductive biology of G. optiva and to develop planned breeding programme for the species.
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