PJB-2018-621
Comparative sequential solvent extraction of phytochemicals from ripe, ripening and unripe Carissa carandas fruit extracts and their antioxidant investigation
Shagufta Irshad, Asia Iqbal, Amna Zulqarnain, Sadia Nawaz and Saadat Anjum
Abstract
Present study was structured to explore the full potential of Carissa carandas fruits whose potential is unidentified in Pakistan. Comparative study of unripe white, ripening pinkish and fully ripe reddish-purple fruit was commenced with sequential solvent extraction (10g/100 mL) by following aqueous (aq.), methanol, ethyl acetate, chloroform and n- hexane to investigate maximum phytochemicals. The results showed progressive extraction in methanol and aq. extracts as both were observed rich in phytochemicals. The high total antioxidant contents were found 65 and 105 mg/g in aq. and methanol extract of ripe fruit respectively as compared to unripe and ripening extract fractions. Percentage of radical scavenging activity (%RSA) was found maximum of 76 and 74% for 50 µL aq. fractions of ripening and unripe fruits respectively while 100 µL aq. ripe fruit extract showed maximum 44% RSA. Whereas 200 µL of ripe fruit methanol extract showed 83% high RSA while 250 µL of ripening fruit exhibited 78% RSA. The maximum 45% RSA was obtained with 250 µL of unripe fruit methanol extract. Significantly high 88% of hydroxyl radical scavenging activity (%HRSA) was observed with 200 µL of ripening fruit aq. extract, 70% for ripe and 43% for unripe fruit was obtained with 50 µL extract dilutions. Highest %HRSA was calculated for 100 µL ripening fruit methanol extract although 69 and 66 % was estimated for 50 µL of ripe and un- ripe fruit extracts. Thus, ripe fruit showed best results in phytochemical analysis having good antioxidant content and highest antioxidant activity.
To Cite this article:
Irshad, S., A. Iqbal, A. Zulqarnain, S. Nawaz and S. Anjum. 2021. Comparative sequential solvent extraction of phytochemicals from ripe, ripening and unripe Carissa carandas fruit extracts and their antioxidant investigation. Pak. J. Bot., 53(2): DOI: http://dx.doi.org/10.30848/PJB2021-2(36)
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