PJB-2018-624
DEVELOPMENT OF SNP BASED GENETIC IDENTIFICATION KEY FOR DATE PALM (PHOENIX DACTYLIFERA L.)
Nadia Faqir
Abstract
Date palm has a long history of cultivation and a valuable germplasm with little knowledge about genetic makeup and diversity. The objective of this work was to develop a DNA sequence based genetic identification system for date palm cultivars. More than 2.5 kb of DNA fragments of rbcL, atpB, and 16S rRNA genes of chloroplast genome and twelve DNA fragments from nuclear genome of seven cultivars were sequenced. Analysis indicated that three fragments had higher marker index (MI) of 4.61, 3.61 and 2.26 with eight, seven and five SNPs respectively. Eight novel SNPs sites were also found in the sequenced fragment. Thus an SNP typing system was developed for varietal identification of date palm cultivars through unique SNPs signatures. To validate this system, four highest SNPs bearing fragments were sequenced from 30 more date palm varieties. The SNPs data from these four fragments were able to resolve 70% of the varieties with unique signatures. This strategy could be expanded to search more SNPs sites to achieve absolute resolution for identification of date palm germplasm.
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