PJB-2018-630
PROTEIN PROFILING IN PAKISTANI WHEAT VARIETIES
Sadaf Iqbal
Abstract
The main cereal crop responsible for 60% and above proteins to the occupants of this planet Earth is Wheat (Triticum aestivum L.). Its bread making feature makes it the principal food for the inhabitants of the biosphere. This attribute is controlled by seed storage proteins. To evaluate diversity assessment via Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE), 66 varieties of wheat for endosperm storage proteins were collected from three provinces of Pakistan. It was executed by means of 12% running gel and 4% stacking gel. Bivariate data matrix was created for the provinces of Sindh, KPK and Punjab by marking 1/0 where 1 was thought of as present while 0 showed absence. In 27 genotypes from Punjab, 61 alleles were noted generating an average of 2.2 alleles for each genotype. In Sindh, 49 alleles were noted within 15 genotypes providing an average of 3.2 alleles for each genotype. In KPK, 48 alleles were scored in 26 genotypes contributing an average of 1.8 alleles for every genotype. A dendrogram of protein bands was generated for cluster analysis. According to cluster analysis, wheat variety Bhittai was found to be most diverse variety in the province of Sindh while in KPK, DN lines showed maximum similarity among them. AS-02 was found to be most diverse wheat variety in Punjab and had maximum dissimilarity with the other genotypes being studied. Varieties with wide-ranging genetic base must be used to overcome decreased wheat yield production in upcoming years.
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