PJB-2018-668
Primary investigation of the diversity and distribution characteristics of Trichoderma spp. in the specific soil of volcanic forest park and volcano platform
Yuan Chang, Rui Miao, Abdul Majeed Baloch, Zhihong Yao, Abdul Wahid Baloch, Chuanying Jiang, Zhihua Liu and Rongshu Zhang
Abstract
Trichoderma spp. are used in biological plant protection as biofungicides as well as in bioremediation. Trichodrma spp. are also utilized as bio-fertilizer to improve and amend soil. To obtain more optimized germplasm resources of Trichodrma spp., the diversity and distribution of Trichoderma species were investigated and explored in the Volcanic Forest Park and volcanic platform in Heilongjiang Province, China. Twenty-eight soil samples near the root, including the rhizosphere of different plants were collected. The Trichoderma strains were screened by dilution plate method. Species were identified through morphological characteristics, analyses of Internal Transcribed Spacer (ITS) sequences and genetic relationships based on phylogenetic tree. As a result, a total of 201 Trichoderma strains were isolated from the two sampling spots and they were identified to be members of 13 known species of Trichoderma, including T. harzianum, T. hamatum, T. atroviride, T. citrinoviride, T. virens, T. koningii, T. koningiopsis, T. viride, T. tomentosum, T. petersenii, H. stellate, T. saturnisporum and T. asperellum. Among all the identified species, T. harzianum was the dominant species, accounting for 43.28% of the total, followed by T. viride, which accounted for 14.93%. 12 species were obtained from the soil of the Volcanic Forest Park, while 8 species were acquired from the volcanic platform, which showed the difference of species diversity of Trichoderma between the two spots. In addition, there were differences in Trichoderma species and stains according to the differences of plants at each sampling spot. In short, our results would illustrate the elementary diversity and distribution characteristics of Trichoderma spp. in the two specific types of soil and provide novel germplasm resources for improved mixed Trichoderma bio-fertilizer.
To Cite this article:
Chang, Y., R. Miao, A.M. Baloch, Z. Yao, A.W. Baloch, C. Jiang, Z. Liu and R. Zhang. 2020. Primary investigation of the diversity and distribution characteristics of Trichoderma spp. in the specific soil of volcanoic forest park and volcano platform. Pak. J. Bot., 52(1): DOI: http://dx.doi.org/10.30848/PJB2020-1(17)
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