PJB-2018-688
BALB/c mice model of intranasal Aspergillus niger spores exposure and allergic sensitization
Muneeba Khan
Abstract
Fungi are ubiquities and their spores are commonly detected in aerbiological surveys. Most of the plant pathogenic fungi do not cause infections in humans. Exposure to the fungal spores may cause allergic rhinitis and asthma in atopic subjects. Aspergillus sp. has been reported frequently in various aerobiological studies throughout the world including Pakistan. In the presented study, murine model (Balb/c mice) was developed to check the allergenic potential of Aspergillus niger. Test mice groups were sensitized through nasal exposure to purified fungal spores. Our study revealed that the mice showed allergy symptoms, after intranasal sensitization through fungal spores, including puffiness of eyes; sneezing; itching; and decrease in physical activity. Blood analysis of the sensitized mice showed positive allergy test. Total WBC count was done and it was observed that in test mice eiosinophils count was significantly higher (p-value > 0.01) as compared to control mice. Statistical analysis was done by using Systat Software, Inc. SigmaPlot for Windows ver. 13.0. Correlation of leucocytes count of control and test group was done by using two tailed t-test. The findings strongly suggested that the spores of Aspergillus niger were potent aeroallergen that are capable eliciting allergic reaction.
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