PJB-2018-692
DIRECT AND INDIRECT PLANTLET REGENERATION FROM EXPLANTS OF ALBIZIA (ALBIZZIA FALCATARIA)
Sukendah
Abstract
Albizia (Albizzia falcataria) is an industrial raw material, used in paper and plywood. Usually farmers generate their own seedlings; however, they have a great variability in terms of growth and wood quality. The research described here developed plantlets through direct and indirect organogenesis of selected explants. Sterile seeds were taken from albizia mother plants and cultured in hormone-free Murashige & Skoog (MS) medium to generate explants. Three kinds of sterile protocol were tested. Nodal segments were cultured in MS basal medium containing 1,2 or 3 mgL-1 6-benzylaminopurine (BAP) for direct plantlet regeneration. Indirect plantlet regeneration through callus formation was done by culturing leaf, stem and root explants in MS basal medium containing 2 mgL-1 2,4-dichlorophenoxyacetic acid (2,4-D). No explants were contaminated and about 98% of explants remained sterile and viable from seeds that were sterilized in 30% sodium hypochlorite for 20 minutes, 70% alcohol for 5 minutes, 20% sodium hypochlorite for 20 minutes and 5% sodium hypochlorite (Protocol II technique of sterilization). The best concentration of BAP for direct planlet regeneration was 2 mgL-1. Callus formation could be induced from stem, leaf and root explants; however, the largest amount of morphogenic callus was formed from stem explants. Albizia morphogenic callus had a friable and nodular structure, compact, shiny, with yellowish colour. This morphogenic callus regenerated into plantlets. Complete plantlets from direct and indirect in vitro regeneration were ready for acclimatization.
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