PJB-2018-709
In vitro biological evaluation and DNA damage protection activities of Cotoneaster afghanicus G.Klotz and Tamarix arceuthoides Bunge extracts
Shazia Anwer Bukhari, Ghulam Mustafa, Shahzad Bashir, Muhammad Farrukh Tahir and Fozia Anjum
Abstract
Two medicinal plants i.e. Cotoneaster afghanicus G.Klotz and Tamarix arceuthoides Bunge were collected from Quetta and Ziarat valleys of Province Balochistan, Pakistan. These plants were selected because their medicinal and nutraceutical effects were not reported before. n-hexane and chloroform extracts of C. afghanicus and T. arceuthoides were subjected to find antibacterial, thrombolytic, biofilm inhibition and DNA damage protection activities. Extracts from both plants showed antibacterial activity by forming inhibition zones of different sizes. Strong antibacterial effect was observed against Micrococcus spp. (source: poultry farm dust samples) which showed some resistance against antibiotic erythromycin used as standard but n-hexane extract of T. arceuthoides showed the highest activity of 27.25 ± 0.37 mm among the extracts. Biofilms are the external matrix of proteins and polysaccharides secreted by bacteria. This matrix provides extra protection to bacteria and therefore, difficult to eradicate. One Gram-positive and one Gram–negative bacterial strain were used to test biofilm inhibition activity. Both n-hexane and chloroform extracts of C. afghanicus showed prominent biofilm inhibition activity against S. aureus with a % inhibition of 59.72 ± 1.98% and 62.03 ± 1.83%. C. afghanicus n-hexane extract exhibited considerable thrombolytic effect of 52.92% clot lysis. Compounds having good antioxidant activities showed DNA damage protection present in higher amounts in n-hexane extract of T. arceuthoides
To Cite this article:
Bukhari, S.A., G. Mustafa, S. Bashir, M.F. Tahir and F. Anjum. 2021. In vitro biological evaluation and DNA damage protection activities of Cotoneaster afghanicus G.Klotz and Tamarix arceuthoides Bunge extracts. Pak. J. Bot., 53(1): DOI: http://dx.doi.org/10.30848/PJB2021-1(15)
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