PJB-2018-713
LIGNIN CONTENT CORRELATION TO LIGNIN-DEGRADATION ENZYME ACTIVITY AND GERMINATION RESPONSES OF HAWTHORN SEEDS DURING COLD STRATIFICATION
bao jian ping
Abstract
Crataegus songarica K. Koch is a critically endangered plant known only in Yili and Hecheng county, Xinjiang Uygur autonomous region, china. Because of the rapid economic development, population growth, pollution, and continuing resource exploitation, Hawthorn faces severe threats. Seed propagation is the main reproduction form, but hawthorn seeds dormancy have an obstacle to germination. We report the influence of cold stratification treatments before germination on hawthorn seed germination and its germinability is associated with a loss of lignin content or changes in degraded enzyme activities of degraded lignin. The results indicated that germination percentage has clearly increased with the increasing time of cold stratification, the maximum germination was 72.16% at 700 days. Lignin content, the main component for seed hardness coat, decreased significantly at cold stratification. Enzyme (LiP, MnP and laccase) activities of degraded lignin was a good indicator of lignin content in hawthorn seeds. LiP, MnP and laccase activities of hawthorn seeds firstly increased and then declined constantly during the cold stratification, the highest enzyme activities of LiP, MnP and laccase was obtained at 700 days of cold stratification. The results of this study showed that cold stratification could effectively break dormancy of hawthorn seeds. The mechanisms’ responsibility for this outcome are a lower level of lignin and a higher level of activity of degraded lignin enzymes.
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