PJB-2018-723
TRANSCRIPTOME ANALYSIS OF GENES RELATED TO CADMIUM ABSORPTION AND TRANSPORTATION IN PEPPER
Zhuqing Zhang
Abstract
Heavy metal pollution is becoming a serious problem in soil contamination in the global. Cadmium (Cd) is the ubiquitous environmental toxic pollutant to plants, which has a greatly affects in the growth and development of plants and even lead to death. In this study, a comparative transcriptome analysis was performed to identify the regulators and pathways that are involved in the cadmium absorption and transportation in the roots, leaves and fruits of pepper in the wild type and mutant. 883,591943 million reads were mapped to the reference genome and 33927 genes were identified in total, reads of each sample and the reference genome alignment efficiency was between 78.99% ~ 84.57%. Finally, 798 DEGs in the roots, leaves and fruits of the mutant and wild type were identified totally, 80 DEGs were up-regulated and 718 DEGs were down-regulated in the 798 DEGs. The DGEs were mostly related to the phenylpropanoid biosynthesis pathway and glyoxylate and dicarboxylate metabolism. In addition, some vital DEGs of them were identified in absorption and transportation like ABC transporter and P-type ATPase. The results revealed several genes underlying the regulation of cadmium absorption and transportation, it may provide a novel insight in the future research and lay the foundation for the molecular mechanism of cadmium absorption and transport in pepper.
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